Part of the new north-south cycle superhighway along Blackfriars Road opened on Thursday morning.
Now cyclists can use the segregated bike track in both directions as far north as the Post Office in Blackfriars Road.
Work on the cycle superhighway project started ten months ago.
Contractors are currently working on the section of Blackfriars Road between Meymott Street and Stamford Street. A new Santander Cycles docking station will be installed on the central reservation between the cycle superhighway and the general traffic lanes.
Later this year the route will extend as far north as Farringdon Street and will eventually reach King's Cross.
Earlier this week local residents were surprised to see part of the new bike lane in Blackfriars Road being dug up even before it opened.
"A 10-metre section of the North-South Cycle Superhighway has been resurfaced after engineers noticed pools of water following recent heavy rain," said Nick Fairholme, director of projects and programmes in Transport for London's surface transport division.
"This was an isolated drainage problem and has been corrected by the contractors at no additional cost to TfL."
The new route features several new two-stage turns for cyclists. TfL has produced a video explaining how to use these junctions.