The new Tesco Express store in Tower Bridge Road opened its doors on Wednesday.
The Lloyds TSB cash machine has been retained outside the shop.
Tower Bridge Road already has many convenience stores, including a 24-hour Costcutter adjacent to Tesco Express.
There is also a Tesco Express store at Empire Square in Long Lane and another branch recently opened in the former Woolworths premises at The Blue in Southwark Park Road.
Tesco Express Tower Bridge is open daily 6am-11pm.