The operators of the Coronet club at Elephant & Castle are appealing to the site's owners to ensure the historic venue has a future when the shopping centre is redeveloped.
The existing 1932 structure – which boasts Art Deco interiors – incorporates fragments of an 1879 building and was placed on the theatres at risk register by the Theatres Trust last autumn.
The building was used as a theatre until 1928 and remained open as a cinema until 1999. Since 2003 it has functioned as a nightclub and music venue.
The current operators say that they provide full and part-time employment to more than 100 people and bring more than 250,000 visitors to the Elephant each year.
New owners Delancey and APG are planning to replace the shopping centre with a new 'town centre' including hundreds of new homes.
"It is still early days and we are just beginning the listening exercise, asking the community what it is they would like to see," said Delancey in a statement.
"Of course, we want to hear from all of our tenants and their future aspirations and needs.
"We have always been clear that the best way to achieve the full regeneration of the site is to redevelop the shopping centre including the Coronet site.
"However, there are no definitive plans for the Coronet with whom dialogue continues and, of course, the planning process is long and we are here to ask the questions and listen to the answers."