Itinerant ice cream selling has now been banned in Southwark's riverside streets from Oxo Tower Wharf in the west to St Saviour's Dock in the east.
An earlier attempt to implement the ban was thwarted when the council failed to follow the proper procedures.
Residents in the Bankside area have been complaining about noise and nuisance from ice cream vans and other traders for at least 10 years.
Licensed street busking will be confined to a spot just west of the Millennium Bridge.
Southwark Council is inviting applications from street traders who wish to operate on Bankside. A six-month pilot begins on 1 December.
Income from the new licenced pitches will fund the enforcement on the ban on itinerant ice cream trading.
Traders interested in applying for the six-month licence should register their interest with email@example.com by Friday 5 November.