The first phase of the North-South Cycle Superhighway was opened to the public on Thursday when the barriers came down on the section from Blackfriars Bridge to Farringdon Street.
In 2011 cycling campaigners staged a series of protests at the bridge linked to City of London plans to remodel the junction at the northern end.
Cycle superhighway 6 from St George's Circus to Farringdon Street has taken more than a year to build.
Transport for London recently held a public consultation on plans for the next phase of the route between Farringdon Street and King's Cross.
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